The main focus of my research is the application of hyperpolarization to the MRI (Magnetic Resonance Imaging) cancer imaging, with the specific focus on the parahydrogen-based techniques such as PHIP (Para-Hydrogen Induced Polarization) and SABRE (Signal Amplification By Reversible Exchange).
1. Development and Scale-Up Synthesis of Hyperpolarized (HP) Tracers and their precursors labeled with 2H (D), 13C and 15N for Real Time Metabolic MRI (Magnetic Resonance Imaging). Phosphoenolpyruvate-1-13C-d2 for production Hyperpolarized (HP) lactate and vinyl acetate-1-13C for the production of HP acetate are only a few examples of this methodology.
2. Optimization of ultrafast Molecular Hydrogenations tailored to the efficient Polarization Transfers in PHIP (Para-Hydrogen Induced Polarization) methodology. The structure optimization of PHIP catalyst aimed at higher polarization levels as well as purer final HP tracer is the main focus of this research direction.
3. Development and Optimization of novel directions in SABRE (Signal Amplification By Reversible Exchange) Hyperpolarization methodology. SABRE polarization of isotopically enriched (15N) nicotinamide-1-15N, as well imidazole-1-15N2 (potential in vivo pH tracer), is one of the recent advances of this research direction.
Shchepin R.V., Barskiy D.A., Coffey A.M., Manzanera Esteve I.V., Chekmenev EY. Efficient Synthesis of Molecular Precursors for Parahydrogen-Induced Polarization of Ethyl Acetate-1-13C and Beyond. Angew. Chem. Int. Ed. (accepted)
Shchepin, R.V., Truong, M.L., Theis, T., Coffey, A.M., Shi, F., Waddell, K.W., Warren, W.S., Goodson, B.M. and Chekmenev, Eduard Y. Hyperpolarization of Neat Liquids by NMR Signal Amplification by Reversible Exchange. J. Phys. Chem. Lett. 6, 1961-1967 (2015).
Theis, T., Truong, M.L., Coffey, A.M., Shchepin, R.V., Waddell, K.W., Shi, F., Goodson, B.M., Warren, W.S. and Chekmenev, E.Y. Microtesla SABRE Enables 10% Nitrogen-15 Nuclear Spin Polarization. J. Am. Chem. Soc. 137, 1404-1407 (2015).
Shchepin, R.V., Coffey, A.M., Waddell, K.W. & Chekmenev, E.Y. Parahydrogen Induced Polarization of 1-13C-Phospholactate-d2 for Biomedical Imaging with >30,000,000-fold NMR Signal Enhancement in Water. Anal. Chem. 86, 5601?5605 (2014). Journal Cover.
Shchepin, R. V.; Coffey, A. M.; Waddell, K. W.; Chekmenev, E. Y., PASADENA Hyperpolarized 13C Phospholactate. J. Am. Chem. Soc., 134 (9), 3957-3960 (2012).